Yes. Absolutely. The tuning has an enormous part in what we hear but the subject is rather ignored except in Baroque music.
In particular the keys of F minor and C minor are particularly sadder than the saddest that equal temperament can convey. This is because the C to Eb and F to Ab are narrow, so making the minor third sadder, and the Eb to G and Ab to C wide, making it a little uncomfortable. Perhaps on
https://jungleboffin.com/mp4/jill-c...-fortepiano/mozart-twinkle-jill-crossland.mp3 you might hear the change from the major to the minor and perhaps it might be a little sadder and
https://jungleboffin.com/mp4/jill-crossland-unequal-tempered-fortepiano/mozart-fantasia.mp3 perhaps might be a little darker. How uncomfortable the minor depends on how strong you like your curry. In contrast A minor sounds as though the sun shines out of it.
The keys developed a reputation for different characteristics and exploiting them was a language in common currency in the 19th century.
I first twigged when listening to Chopin's 2nd Sonata - the final movement being the wind howling cold over the desolate graves and realising that the key of Bb minor was the key in which that would happen particularly as a result of my knowledge of organ tuning.
But others have got there before me - piano technician Ed Foote was working with pianist Enid Katahn 20 - 25 years ago and their recordings are really worth hearing. Look them up and you'll be able to download the CD but one has been uploaded to YouTube
Here's the 2nd Sonata
It's subtle but can be transformatory.
It can also bring extraordinary clarity to the sound
Perhaps you might know the Brahms violin sonatas - this recording
was the first time that it sent me into a trance overlooking the lake at Tun seeing the mirror smooth lake, flurries of leaves at the shore and birds flying overhead.
And here's a piece where the different relationship between notes provides sort of landmarks that perhaps we can recognise as we travel through the piece
https://www.youtube.com/watch?v=oMHvl1yH1pw
I've been working on this for now a dozen years or more and our seminar results from patient, tolerant and now excited musicians coming together. Tuning the piano as an instrument to get reliable results has taken a decade to really understand and now to get repeatable results.
Some of our recordings are now quoted in academic theses, particularly the Chopin 24 preludes recorded in unequal temperament
https://www.youtube.com/watch?v=qdsFLIo9l88
https://www.youtube.com/watch?v=A34K-fj5nHs
https://www.youtube.com/watch?v=XpqrynlohR4
When we go back before the 19th century we have to explore meantone temperament and this is revelationary for Mozart
https://www.academia.edu/37951978/T..._Fantasias_K594_and_K608_for_Mechanical_Clock
When we put the Fantasias through a small barrel organ appropriately tuned, they convey all and more emotion than as performed in modern times with big organs in equal temperament.
And then when we look at early 19th century music through the lens of meantone, it can be as an X-Ray into the music here
https://www.youtube.com/watch?v=oV0bkcSr_Kg bringing the supernatural influences of Shakespeare's "The Mysterious Isle" into open display, particularly documented well in the page in Italian
https://it.wikipedia.org/wiki/La_tempesta - use Google Translate. Suddenly Beethoven's documented but formerly doubted intention is revealed.
Best wishes
David P